In order to investigate the haematopoietic effects of Rehmanniae Radix Preparat various experiments about hematopoiesis were studied.
The results were obtained as follows.
1. In the proliferous effect of bone marrow cells, water extracts of Rehmanniae Radix Preparat played an important role on the proliferating bone marrow cells compared with control group at low density (1 ㎍/㎖).
2. The water extracts of Rehmanniae Radix Preparat activated the bone marrow cells.
3. The water extracts of Rehmanniae Radix Preparat promoted the proliferation of the bone marrow cells into cluster, BFU-E, CFU-GEMM. Particularly, into cluster and CFU-GEMM.
4. In the bone marrow cells treated with CTX, the water extracts of Rehmanniae Radix Preparat promoted the SCF, IL-4, GM-CSF gene expression, but gene expression of its receptors, c-kit, EPO-R, TRO, c-mpl, EKLF, GATA-1 were not promoted significantly.
According to the above results, water extracts of Rehmanniae Radix Preparat promoted not only the differentiation of the bone marrow cells into CFU-GEMM which were mother cell of erythrocytes, thrombocytes, monocytes and macrophages, but also the differentiation of the bone marrow cells into BFU-E. On the other hand, in the gene expression that have a lot of haematopoietic effects in the early differentiation of haematopoietic stem cells by promoting the gene expression of SCF, IL-4, GM-CSF and so forth, Above results also demonstrates that the water extracts of Rehmanniae Radix Preparat have somewhat meaningful effects in hematogenesis but not sufficient to explain its mechanisms about hematogenesis, therefore further deep studies shoud be accomplished about its mechanisms.
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